The development of new approaches to standardization of Cassia acutifolia leaves

There were substantiated the expediency of the using for the determination of identity of Cassia acutifolia Del. leaves of spectrophotometry and TLC. As the results of the chemical study of Cassia acutifolia leaves there were isolated the predominant components of this plant 1,7-dihydroxy-3carboxyanhraquinone, 8-O-β-D-glucopyranoside of torachrysone, kaempferol-3-O-gentiobioside and on this basis the methodological approaches for standardization of Cassia acutifolia leaves were developed. These methodological approaches consist in the determination not only of anthracene derivatives, but also other diagnostic phenolic compounds, including flavonoids and naphthalenes. There was developed the method of quantitative determination of the total anthracene derivatives by means of spectrophotometry at analytical wavelength 530 nm. The contents of the total anthracene derivatives in Cassia acutifolia leaves are varied with 1,21% to 1,88% (calculated on sennoside B).